Our Dengue Fever Virus recombinant proteins include N-terminal His-tagged DIII Envelope proteins for all four subtypes (expressed in E.coli)
Dengue virus is an arthropod-borne avivirus virus.
Four antigenically distinct serotypes (1 to 4) cause a wide range of diseases, from mild dengue fever to severe dengue hemorrhagic fever, and dengue shock syndrome.
It has been estimated that more than 2.5 billion people in over 100 countries are at risk of dengue infection with several hundred-thousand cases of life threatening infections occurring every year.
The avivirus genome encodes three structural proteins: the capsid protein, pre-membrane protein, and envelope protein; and seven non-structural proteins:
The envelope protein comprises three regions: Domain I, Domain II, and Domain III. Domain I is the central domain, Domain II is the dimerization domain, while Domain III (DIII) is an immunoglobulin-like domain.
Experimental evidence has shown that DIII protein is a receptor recognition- and binding domain which has been demonstrated to be highly immunogenic and is able to elicit the production of neutralizing antibodies against wild-type virus.
Consequently, the DIII protein is an important immunogen for the development of a possible protein subunit vaccine as well as a potential diagnostic reagent for improved clinical diagnosis of aviviral infections.